Thermoresponsive micropatterned substrates for single cell studies.

Thermoresponsive micropatterned substrates for single cell studies.

Blog Article

We describe the design of micropatterned surfaces for single cell studies, based on thermoresponsive polymer Zipper Pouch brushes.We show that brushes made of poly(N-isopropylacrylamide) grafted at high surface density display excellent protein and cell anti-adhesive properties.Such brushes are readily patterned at the micron scale via deep UV Hockey Protective - Shoulder Pads - Youth photolithography.A proper choice of the adhesive pattern shapes, combined with the temperature-dependent swelling properties of PNIPAM, allow us to use the polymer brush as a microactuator which induces cell detachment when the temperature is reduced below [Formula: see text]C.