Thermoresponsive micropatterned substrates for single cell studies.
We describe the design of micropatterned surfaces for single cell studies, based on thermoresponsive polymer Zipper Pouch brushes.We show that brushes made of poly(N-isopropylacrylamide) grafted at high surface density display excellent protein and cell anti-adhesive properties.Such brushes are readily patterned at the micron scale via deep UV Hock